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BMA Lausanne

BMA Lausanne: Fourier-transform IR micro-spectroscopy

The BMA Lausanne is specialized in Fourier-transform Infrared (FTIR) spectroscopy and microscopy. The goal of the FTIR laboratory in Lausanne is to provide information on the alteration in chemical structure of biological samples. It offers services in FTIR spectroscopy and microscopy analysis and chemometric and statistical analysis of IR data.

1) FTIR spectroscopy and microscopy analysis
FTIR is a powerful spectroscopic technique commonly used to identify chemical compounds based on their vibrations in the mid-infrared region of the spectrum. It is a non-invasive and non-destructive chemical analysis technique as samples usually require either none or limited treatment for measurement. FTIR allows analysis of a very wide range of samples: tissues (leaves, stems, roots, flowers, etc.), thin films (e.g. obtained using microtome or by evaporation of solvents), solutions/extracts, powders. FTIR microscopy (FTIRM) and FTIR imaging (FTIRI) are techniques combining light microscopy and IR microscopy. Light microscopy is used to magnify structural detail in samples, while IR spectroscopy provides information on molecular chemistry. Their combination permits chemical analysis in microscopic detail.

2) Chemometric and statistical analysis
Our lab provides support to the plant research community on chemometric analysis of FTIR data of biological samples. Chemometrics is the common name of mathematical and statistical methods focusing on the extraction of relevant information from multidimensional digital data. Chemometrics involves a wide range of data pre-treatment techniques (such as baseline correction, spectra normalization), methods for data treatment (species identification, clustering, quantitative analysis, profiling, etc.) and their visualisation.

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Contact BMA Lausanne

Sylwester Mazurek, Ph.D.

Bio-Molecular Analysis Platform
Université de Lausanne
Département de Biologie
Moléculaire Végétale
CH - 1015 Lausanne
Tel: +41 21 692 42 37
Fax: +41 21 692 41 95
www.unil.ch/dbmv
sylwester.mazurek-at-unil.ch

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CH-1015 Lausanne  - Switzerland  -  Tel. +41 21 69242 62
Swiss University