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Publications des groupes Mermod et Wittek de 2000 à présent.

Droz X, Harraghy N, Lançon E, Le Fourn V, Calabrese D, Colombet T, Liechti P, Rida A, Girod P-A and Mermod N. (2017). Automated microfluidic sorting of mammalian cells for those that efficiently express and secrete a protein of interest. In revision.
Renaud S, Scabia V, Dormoy V, Brisken C, Fiche M, Stravodimou A, Galmiche M, Zaman K and Mermod N. (2017). miR363-3p mediates maintenance and chemoresistance of breast cancer stem cells. Submitted for publication.
Pourcel L, Garcia F and Mermod N. (2017). Use of vitamin B5 transport as a metabolic selection for highly efficient recombinant protein expression by mammalian cells. Manuscript in preparation.

Kostyrko K, Neuenschwander S, Junier T, Regamey A, Iseli C, Schmid-Siegert E, Bosshard S, Majocchi S, LeFourn V, Girod PA, Xenarios I and Mermod N. (2017). MAR-Mediated Transgene Integration into Permissive Chromatin and increased expression involve an SD-MMEJ-like DNA repair Pathway. Biotechnol. Bioeng., 114, 384-396.

Mee ET, Preston MD, CS533 Study Participants, Minor PD, Schepelmann S. (2016). Development of a candidate reference material for adventitious virus detection in vaccine and biologicals manufacturing by deep sequencing. Vaccine, 34, 2035-2043.

Kostyrko K and Mermod N. (2016). Assays for DNA double-strand break repair by microhomology-based end-joining repair mechanisms. Nucl. Acids Res. 44(6): e56.

Van Zwieten RW, Majocchi S, Puttini S, Messina G, Tedesco FS, Cossu G and Mermod N. (2015). MAR-mediated dystrophin expression in mesoangioblasts for Duchenne muscular dystrophy cell therapy. Molec. Biol., 4, 134.

Kostyrko K, Bosshard S, Urban Z, and Mermod N. (2015). A role for homologous recombination proteins in cell cycle regulation. Cell Cycle, 14, 2853-2861.

Harraghy N, Calabrese D, Fisch I, Girod PA, LeFourn V, Regamey A, and Mermod N. (2015). Epigenetic regulatory elements: recent advances in understanding their mode of action and use for recombinant protein production in mammalian cells. Biotechnol. J., 10, 967-978.

Edelmann S, Fahrner R, Malinka T, Song BHC, Stroka D and Mermod N. (2015). Nuclear Factor I-C acts as a regulator of hepatocyte proliferation at the onset of liver regeneration. Liver Int. 35:1185–1194.

Alberti L, Renaud St, Losi L, Leyvraz S, Benhattar J. (2014). High Expression of hTERT and Stemness Genes in BORIS/CTCFL Positive Cells Isolated from Embryonic Cancer Cells. Plos one.

Puttini S, Panaite PA, Mermod N, Renaud S, Steck AJ, and Kuntzer T. (2014). Gene Expression Changes in Chronic Inflammatory Demyelinating Polyneuropathy Skin Biopsies. NeuroImmunology. 270:61-66.

Ley D, Puttini S, van Zwieten R, Iyer P, and Mermod N. (2014). A PiggyBac mediated approach for muscle gene transfer or cell therapy. Stem Cell Res. 13:390-403.

Deak F, Matés L, Korpos E, Zvara A, Kiricsi M, Mendler L, Szénasi T, Zsak Y, Puskas LG, Mermod N, Dux L, and Kiss I. (2014). Extracellular matrilin-2 deposition mediates a timely induction of the myogenic regulatory program. Journal of Cell Science. 127:3240-3256.

Majocchi S, Aritonovska E, and Mermod N. (2014). Epigenetic regulatory elements associate with specific histone modifications to prevent silencing of telomeric genes. Nucleic Acids Research. 42:193-204.

Van Zwieten R, Puttini S, Lekka M, Witz G, Gicquel-Zouida E, Richard I, Lobrinus JA, Chevalley F, Brune H, Dietler G, Kulik A, Kuntzer T, and Mermod N. (2014). Assessing dystrophies and other muscle diseases at nanometer scale by atomic force microscopy. NanoMedicine. 9:393-406.

Le Fourn V, Girod PA, Buceta M, Regamey A, and Mermod N. (2014). CHO cell engineering to prevent polypeptide aggregation and improve therapeutic protein secretion. Metabolic Engineering. 21:91-102.

Bire S, Casteret S, Ley D, Mermod N, Bigot Y, and Rouleux-Bonnin F. (2013). Optimization of piggyBac gene delivery tool using mRNA and insulators. PLoS ONE, 8:e82559.

Puttini S, van Zwieten R, Saugy D, Lekka M, Hogger F, Ley D, Kulik AJ, and Mermod N. (2013). MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation. BMC Mol Biol, 14:26, doi:10.1186/1471-2199-14-26.

Arope S, Harraghy N, and Mermod N. (2013). Molecular characterization of a human matrix attachment region epigenetic regulator. PLoS ONE, 8:e79262.

Pjanic M, Schmid CD, Gaussin A, Ambrosini G, Adamcik J, Pjanic P, Plasari G, Kerschgens J, Dietler G, Bucher P, and Mermod N. (2013). Nuclear Factor I genomic binding associates with chromatin boundaries. BMC Genomics. 14:99.

Ley D, Harraghy N, Le Fourn V, Bire S, Girod P-A, Regamey A, Rouleux-Bonnin F, Bigot Y, and Mermod N. (2013). MAR Elements and Transposons for Improved Transgene Integration and Expression. PloS ONE, 8:e62784.

Contie M, Leger O, Kosco-Vilebois M, Mermod N, and Elson G, (2013). IL-17F Co-Expression Improves Cell Growth Characteristics and Enhances Recombinant Protein Production during CHO Cell Line Engineering. Biotechnology & Bioengineering. 110:1153-1163.

Bire S, Gosset D, Jégot G, Midoux P, Pichon Ch and Rouleux-Bonnin F. (2013). Exogenous mRNA delivery and bioavailability in gene transfer mediated by piggyBac transposition. BMC Biotechnology, 13:75  doi:10.1186/1472-6750-13-75

Mernier G, Majocchi S, Mermod N, and Renaud P. (2012). In situ evaluation of single-cell lysis by cytosol extractionobservation through fluorescence decay and dielectrophoretic trapping time through fluorescence decay and dielectrophoretic trapping time. Sensors and Actuators B:166-167, p. 907-912.

Gaussin A, Modlich U, Niederländer N, Schambach A, Baum C, Auclair C, Cohen-Haguenauer O, and Mermod N. (2012). CTF/NF1 transcription factors act as potent genetic insulators for integrating gene transfer vectors. Gene Therapy, doi:10.1038/gt.2011.70

Harraghy N, Farina M, Regamey A, Girod P-A, and Mermod N. (2012). Using matrix attachment regions to improve recombinant protein production. Methods in Molecular Biology. 801:93-110.

Harraghy N, Regamey A, Girod PA, and Mermod N. (2011). Identification of a potent MAR element from the mouse genome and assessment of its activity in stable and transient transfections. J Biotechnol. 2011 Jun 10;154(1):11-20. doi: 10.1016/j.jbiotec.2011.04.004.

Grandjean M, Girod P-A, Calabrese D, Wicht M, Beckman JS, Martinet D and Mermod N. (2011). High-level transgene expression by homologous recombination-mediated gene transfer. Nucleic Acids Research, 39 (15): e104. doi: 10.1093/nar/gkr436.

Pjanic M, Pjanic P, Schmid C, Ambrosini G, Gaussin A, Bucher P and Mermod N. (2011). Nuclear factor I revealed as family of promoter binding transcription activators using random sampling approach. BMC Genomics, 12:181.

Kerschgens J, Renaud S, Schütz F, Grasso L, Egener-Kuhn T, Delaloye JF, Lehr HA, Vogel H and Mermod N. (2011). Protein-Binding Microarray Analysis of Tumor Suppressor AP2α Target Gene Specificity. Molecular and Cellular Proteomics, Plos One 6(8) p. e22895. 

Buceta M., Galbete JL., Kostic C., Arsenijevic Y. and Mermod N. (2011). Use of human MAR elements to improve retroviral vector production. Gene Therapy, 18:7-13.

Plasari G, Edelmann S, Hogger F, Dusserre Y, Mermod N and Calabrese A. (2010) Nuclear Factor I-C regulates TGF-β-dependent hair follicle cycling. Journal of Biological Chemistry, 285: 34115–34125.

Gaussin A., and Mermod N. (2010). Chromatin insulators and Prospective Application for gene Therapy. Gene Therapy Review. 2(1) online publication.

Plasari G., Calabrese A., Dusserre Y., Gronostajski RM., McNair A., Michalik L., and Mermod N. (2009). Nuclear Factor I-C links PDGF and TGF-b1 signaling to skin wound healing progression. Molec. Cell. Biol. 29:6006-6017.

Kerschgens J., Egener-Khun T., Mermod N. (2009). Protein-binding microarrays: probing disease makers at the interface of proteomics and genomics. Trends in Molecular Medicine, Volume 15, Issue 8, Pages 352-358.

Escher P., Gouras P., Roduit R., Tiab L., Delarive T., Bolay S., Chen,S., Tsai C.-C., Mermod N., Hayashi M., Zernant,J., Merriam J., Allikmets R., Munier F.-L., Schorderet D.-F. (2009). Mutations in NR2E3 can cause dominant or recessive retinal degenerations in a same family. Human Mutation, 30: 342-351.

Gorman C., Arope S., Grandjean M., Girod P.-A., and Mermod N. (2009). Use of MAR elements to increase the production of recombinant proteins. Cell Line Development. Al-Rubeai M., ed. Springer Science. Cell Engineering Vol. 6, pp 1-32.

Fournier T., Gabriel J.-P., Mazza C., Pasquier J., Galbete J.-L., and Mermod N. (2009). Stochastic models and numerical algorithms for a class of regulatory networks. Bull. Math. biol., 71:1394-1431.

Esnault G., Majocchi St., Martinet D., Besuchet-schmutz N., Beckmann JS., and Mermod N. (2009). Transcription factor CTF1 acts as a chromatin domain boundary protein that shields human telomeric genes from silencing. Mol. Cell. Biol, 29:2409-2418.

Galbete J.-L., Buceta M., and Mermod N. (2009). MAR elements regulate the probability of epigenetic switching between active and inactive gene expression. Molec Biosyst, 5:143-150.

Puttini St., Lekka M., Dorchies O.-M., Saugy D., Incitti T., Ruegg U.-T., Bozzoni I., Kulik A.-J., and Mermod N. (2009). Gene-mediate restoration of normal myofiber elasticity in dystrophic muscles. Molecular Therapy, 17:19-25.

Harraghy N., Gaussin A., and Mermod N. (2008). Sustained expression using MAR elements. Current Gene Therapy, 8:353-366.

Fournier T., Gabriel J.-P., Mazza C., Pasquier J., Galbete J.-L. and Mermod N. (2007). Steady-state expression of self-regulated genes. Bioinformatics, 23, 3185-3192

Girod P-A, Nguyen D., Calabrese D., Grandjean M., Martinet D., Beckmann J., Bucher P., and Mermod N. (2007). Genome-wide prediction of matrix attachment regions that increase gene expression in mammalian cells. Nature Methods 4, 747-753.

Karlen Y., McNair A., Perseguer S., Mazza C. Mermod N. (2007). Statistical significance of quantitative PCR. BMC Bioinformatics, 8:131.

Wenzlow N, Plattet P, Wittek R, Zurbriggen A, Gröne A. (2007). Immunohistochemical demonstration of the putative canine distemper virus receptor CD150 in dogs with and without distemper. Vet Pathol.;44(6):943-8.

Brunner J. M., Plattet P., Majcherczyk P., Zurbriggen A., Wittek R., Hirling H., (2007). Canine distempler virus infection of primary hippocampal cells induces increase in extracellular glutamate and neurodegeneration. Journal of Neurochemistry 103(3), 1184-95.

Plattet P., Cherpillod P., Wiener D., Zipperle L., Vandevelde M., Wittek R., Zurbriggen A., (2007). Signal peptide and helical bundle domains of virulent canine distemper virus fusion protein restrict fusogenicity. Journal of Virology 81(20), 11413-25.

Rivals J. P., Plattet P., Currat-Zweifel C., Zurbriggen A., Wittek R., (2007). Adaptation of canine distemper virus to canine footpad keratinocytes modifies polymerase activity and fusogenicity through amino acid substitutions in the P/V/C and H proteins. Virology 359(1), 6-18.

Girod P.-A., Grandjean M., Calabrese D., Martinet D., Beckmann J., Mermod N. (2006). Fast generation of highproducer cho cell lines by an iterative transfection process. BMC Microbial Cell Factories 5:S41.

Wittek R., (2006). Vaccinia immune globulin: current policies, preparedness, and product safety and efficacy.  International Journal of Infectious Diseases 10(3), 193-201.

Vyroubalova E., Hartley O., Mermod N. and Fisch I. (2006). Identification of peptide ligands to the chemokine receptor CCR5 and their maturation by gene shuffling strategy. Molecular Immunology, 43, 1573-1578.

Suarez M., Haenni M., Canarelli S, Fisch F., Chodanowski PP., Servis S., Michielin O., Freitag R., Moreillon P. and Mermod N. (2005). Structure-function characterization and optimization of a plant-derived antibacterial peptide. Antimicrob. Agents Chemother. 49, 3847-3857.

Egener T., Roulet E., Bucher P. and Mermod N. (2005). Proof of concept for microarray-based detection of DNA-binding oncogenes in cell extracts. Nucl. Acids Res. 33, e79.

Girod P-A., Zahn M. and Mermod N. (2005). Use of Matrix attachment regions to generate high producer cell lines. Biotechol. Bioeng. 91, 1-11.

Girod PA., Zahn M. and Mermod N. (2005). MAR elements as tools to increase protein production. In: Animal cell technology meets genomics. Casablancas FA et al., eds. Kluwer Academic Publishing, The Netherlands, pp 411-415.

Plattet P., Rivals J. P., Zuber B., Brunner J. M., Zurbriggen A., Wittek R., (2005). The fusion protein of wild-type canine distemper virus is a major determinant of persistent infection. Virology 337(2), 312-26.

Tan N.S., Michalik L., Di-Poï N., Ng C.Y., Mermod N., Roberts A., Desvergne B. and Wahli W. (2004). Essential role of Smad3 in the inhibition of inflammation-induced PPARβ/δ expression. EMBO J., 23, 4211-4221.

Ferrari S., Carmine-Simmen K., Dusserre Y., Müller K., Fourel G., Gilson E. and Mermod N. (2004). Chromatin domain boundaries delimited by a histone-binding protein in yeast. J. Biol. Chem., 279, 55520-55530.

Cherpillod P., Zipperle L., Wittek R., Zurbriggen A., (2004). An mRNA region of the canine distemper virus fusion protein gene lacking AUG codons can promote protein expression. Archives of Virology 149(10), 1971-83.

Griot C., Moser C., Cherpillod P., Bruckner L., Wittek R., Zurbriggen A., Zurbriggen R., (2004). Early DNA vaccination of puppies against canine distemper in the presence of maternally derived immunity. Vaccine 22(5-6), 650-4.

Plattet P., Zweifel C., Wiederkehr C., Belloy L., Cherpillod P., Zurbriggen A., Wittek R. (2004). Recovery of a persistent Canine distemper virus expressing the enhanced green fluorescent protein from cloned cDNA. Virus Resarch 101(2), pp.147-53.

Wittek R., (2004). Could smallpox come back?  International Journal of Infectious Diseases, 8 suppl. 2, pp.S1.

Briguet A., Bleckmann D., Bettan M., Mermod N., and Meier T. (2003). Transcriptional activation of the utrophin promoter B by a constitutively active Ets transcription factor. Neuromuscul. Disord., 13, 143-150.

Girod P.-A. and Mermod N. Use of Scaffold/Matrix-Attachment Regions for protein production Gene Transfer and Expression in Mammalian Cells. (2003) In: New Comprehensive Biochemistry, Gene transfer and expression in mammalian cells. Makrides S., ed. Elsevier Science. 38, 359-379.

Suarez S., Entenza JM., Doerries C., Meyer E., Marison I., Moreillon P. and Mermod N. (2003). Expression of a plant-derived peptide harboring water cleaning and antibiotic activities. Biotechnol. Bioeng., 81, 13-20.

Meertens N., Stoffel M.-H., Cherpillod P., Wittek R., Vandevelde M., Zurbriggen A. (2003). Mechanism of reduction of virus release and cell-cell fusion in persistent canine distemper virus infection. Acta Neuropathol. 106(4), pp. 303-10.

Roulet E., Busso S., Camargo AA., Simpson AJG., Mermod N. and Bucher P. (2002). High resolution and characterization of transcription factor DNA binding specificity by coupled protocols for SAGE-assisted SELEX and computer modeling. Nature Biotechnol., 20, 831-835.

Mermod N. and Cornetta K. G. (2002). The ins, the outs... and the next generation. Current Opin. Biotechnol., 13, 415-417.

Imhof M. O., Chatellard P., and Mermod N. (2002). Comparative study and identification of eukaryotic transcriptional repressors in gene switch systems. J. Biotechnol., 97, 275-285.

Fourel G., Boscheron C., Revardel E., Lebrun E., Hu Y.-F., Carmine Simmen K., Müller K., Li R., Mermod N., Gilson E. (2001). An activation-independent role of transcription factors in insulator function. EMBO R., 2, 124-132.

Cottet-Maire F., Roulet E., Buetler T.M., Mermod N., Ruegg U.T. (2001). Upregulation of vasopressin V1a receptor mRNA and protein in vascular smooth muscle cells following cyclosporin A treatment. Brit. J. Pharmacol., 132, 909-917.

Zahn-Zabal M., Kobr M., Imhof M.O., Chatellard P., de Jesus M., Wurm F. and Mermod N. (2001). Development of stable cell lines for production or regulated expression using matrix attachement regions. J. Biotechnol. , 87, 29-42.

Griot-Wenk M. E., Cherpillod P., Koch A., Zurbriggen R., Bruckner L., Wittek R., Zurbriggen A. (2001). The humoral immune response to recombinant nucleocapsid antigen of canine distemper virus in dogs vaccinated with attenuated distemper virus or DNA encoding the nucleocapsid of wild-type virus. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine 48(5), pp. 295-302.

Tipold A., Vandevelde M., Wittek R., Moore P., Summerfield A., Zurbriggen A. (2001). Partial protection and intrathecal invasion of CD8(+) T cells in acute canine distemper virus infection. Veterinary Microbiology 83(3), pp. 189-203.

Miescher S., Zahn-Zabal M., De Jesus M., Moudry R., Fisch I., Vogel M., Kobr M, Imboden M.A., Kragten E., Bichler J., Mermod N., Stadler B.C., Amstutz H., Wurm F. (2000). CHO  Expression of a Novel Human Recombinant IgG1 anti-Rh D Antibody Isolated by Phage Display. Brit. J. Haematol., 111, 157-166.

Roulet E., Bucher P., Schneider R., Wingender E., Dusserre Y., Werner T. and Mermod N. (2000). Experimental analysis and computer prediction of CTF/NFI Transcription Factor DNA binding sites. J. Mol. Biol. 297, 833-848.

Imhof M. O., Chatellard P., and Mermod N. (2000). A regulatory network for efficient control of transgene expression. J. Gene. Med. 2, 107-116.

Müller K. and Mermod N. (2000). The histone-interacting domain of Nuclear Factor One activates SV40 DNA replication. J. Biol. Chem., 275, 1645-1650.

Cherpillod P., Tipold A., Griot-Wenk M., Cardozo C., Schmid I., Fatzer R., Schobesberger M., Zurbriggen R., Bruckner L., Roch F., Vandevelde M., Wittek R., Zurbriggen A., (2000). DNA vaccine encoding nucleocapsid and surface proteins of wild type canine distemper virus protects its natural host against distemper. Vaccine 18(26), pp. 2927-36.



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