Direct Arterial (invasive)
This method allows for direct arterial blood pressure measurement in the awake, conscious animal after surgery.
A heparined catheter is placed into the carotid artery of the anesthetized rodent and tunneled subcutaneously to extrude at the back of the neck. Anywhere from 3 to 6 hours is scheduled for recovery from surgery. The blood pressure is recorded following a 1/2 hour adaptation period after connection to the recording equipment. Measurements are acquired with Hem Software (Notocord Systems, Croissy, France) and associated pressure and ECG amplifier systems.
Recording of Blood pressure in 4 mice
Non-invasive blood pressure
This method allows a measurement of non-invasive blood pressure in the awake, conscious animal.
Non-invasive blood pressures are obtained from the mice using the Blood Pressure Analysis System from Visitech Systems, Inc (Apex, NC). The pulse is detected on the tail, distal to the tail cuff, with a photoelectric sensor. The detected pulse is displayed on the computer monitor and the cuff is inflated by the system. The mice are placed on a warm platform to increase blood flow to the tail in order to improve the pulse detection. The mice are trained on the machine for 5 consecutive days, and the final values are obtained on the sixth and seventh day (unless more days are requested).
This method allows for long term direct arterial blood pressure measurement in the awake, conscious animal after surgery.
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This method allows for direct measurement of left ventricle pressure, Contractility Index and PV loop analysis in animal after surgery.
A 1Fr catheter (Millar) is placed into the right carotid artery of the anesthetized rodent until entering in the left ventricle. Measurements are acquired with IOX Software (EMKA Technologies, France) and associated Millar amplifier systems MPVS Ultra Single Segment.