Cardiac dimensions, function, and Doppler
The platform has a high frequency ultrasound machine specifically manufactured for use in mice and rats. This system was developed by VisualSonics in Toronto, Canada. The probes range from 30 to 80 MHz with very shallow focal lengths, allowing excellent visualization (around 50 microns). In particular, the analyses of cardiac dimensions (wall thickness, chamber width, etc.) along with cardiac function (ejection fraction, fractional shortening) can be rapidly obtained. All non-invasive transthoracic cardiac ultrasound in performed under light general isoflurane anesthesia.
Long axis view of a mouse heart M-Mode measurements
Long axis view of a mouse heart
Pulsed-Wave Doppler Mode allows us to quantify the flow through the mitral or tricuspid valve
Tissue Doppler Mode allows us to track the motion of tissue, as mitral annulus
Mitral Flow Mitral Annulus Velocity
Tumor surrounding an adrenal gland Three-dimensional view of a tumor (blue) into a kidney (red)