ESTScan is a program that can detect coding regions in DNA sequences, even if they are of low quality. ESTScan will also detect and correct sequencing errors that lead to frameshifts.
ESTScan is not a gene prediction program , nor is it an open reading frame detector. In fact, its strength lies in the fact that it does not require an open reading frame to detect a coding region. As a result, the program may miss a few translated amino acids at either the N or the C terminus, but will detect coding regions with high selectivity and sensitivity.
ESTScan uses a Markov model to represent the bias in hexanucleotide usage found in coding regions relative to non-coding regions. Additionally, ESTScan allows insertions and deletions when these improve the coding region statistics.