Online sites at Science-stke (Signal transduction knowledge Environment):
Liechti, R., Gfeller, A. and Farmer, E.E. The jasmonate signaling pathway Connections Map. First version published August 2004 http://stke.sciencemag.org/cgi/cm/CMP_13820
Liechti, R., Gfeller, A. and Farmer, E.E. The Arabidopsis jasmonate signaling pathway Connections Map. First version published August 2004 http://stke.sciencemag.org/cgi/cm/CMP_13931
Mueller, M.J., Mène-Saffrané, L., Grun, C., Karg, K. and Farmer, E.E. (2006) Oxylipin analysis methods. Plant J. accepted
Armand, F., Bucher, P., Jongeneel, C.V., and Farmer, E.E., (2005) Rapid and selective surveillance of Arabidopsis thaliana genome annotations with Centrifuge. Bioinformatics, 21, 2906-2908.
Schulze-Lefert, P., and Farmer, E.E. (2005) Biotic interactions: From molecular networks to interorganismal communities (Editorial overview). Curr. Opin. Plant Biol. 8, 343-345.
Nagpal, P., Ellis, C.M., Weber, H., Ploense, S., Barkawi, L.S., Guilfoyle, T.J., Hagen, G., Alonso, J.M., Cohen, J.D., Farmer, E.E., Ecker, J.R. and Reed, J.W. (2005) Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation. Development, 132, 4107-4118.
Gfeller, A., Farmer, E.E, (2004) Keeping the Leaves Green Above Us. Science. 316, 1515-1516.
Bariola, P.A., Retelska, D., Stasiak, A., Kammerer, R.A., Fleming, A., Hijri, M., Frank, S. and Farmer, E.E. (2004) Remorins form a novel family of coiled coil-forming oligomeric and filamentous proteins associated with apical, vascular and embryonic tissues in plants. Plant Mol. Biol. 55: 579-594. Identifies a potentially useful marker for lipid rafts.
See videos of remorin localisation.
Previous publications on remorin from our work :
Reymond, P., Bodenhausen, N., Van Poecke, R.M.P., Krishnamurthy, V., Dicke, M. and Farmer, E.E. (2004) A conserved transcript pattern in response to a specialist and a generalist herbivore. Plant Cell, 16, 3132-3147.
Demonstrates that between 67 to 84 % of insect activated gene expression depends, directly or indirectly on the jasmonate signal pathway.
Weber, H., Chételat, A, Reymond, P. and Farmer, E.E. (2004) Selective and powerful stress gene expression in Arabidopsis in response to malondialdehyde. Plant J. 37, 877-888.
Demonstrates that malondialdehyde(MDA) can have potent biological activtiy at low concentation in an intact eukaryote thus confirming predictions made in Alméras et al.
Farmer E.E, Almeras E. and Krisnamurthy V., (2003) Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory. Curr. Op. Plant Biol 6, 372-378.
Alméras E., Stolz S., Vollenweider S., Reymond P., Mène-Saffrané L., and Farmer, E.E., (2003) Reactive electrophile species activate defense gene expression in Arabidopsis. Plant J. 34, 205-216. Shows that the ubiquitous non-enzymatic lipid oxidation product MDA can regulate survival and defense genes
Liechti, R., Farmer, E.E., (2002) The jasmonate pathway. Science 296 (5573): 1649-50. Review
Weber, H. (2002) Fatty acid-derived signals in plants. TRENDS in Plant Science 7, 217-224
Stintzi, A., Weber, H., Reymond, P., Browse, J. and Farmer, E.E. (2001) Plant defense in the absence of jasmonic acid: the role of cyclopentenones. Proc. Natl. Acad. Sci. USA, 98, 12837-12842.
commentary by Gregg A. Howe
Confirms that the oxylipin signature concept is correct and shows that OPDA and dinor-OPDA are the key compounds necessary for resistance to an insect and a pathogen. Suggests that cyclopentenone jasmonates might have some electrophilic properties in vivo.
Vollenweider, S., Weber, H., Stolz, S., Chételat, A. and Farmer, E.E. (2000) Fatty acid ketodienes and fatty acid ketotrienes : Michael addition acceptors that accumulate in wounded and diseased Arabidopsis leaves. Plant J. 24, 467-476.
We show that a variety of pathogenesis-associated lipids including fatty acid ketotrienes activate the GST1 gene in Arabidopsis and that this is structure-dependent.
The structure in question is the a,b-unsaturated carbonyl group. Introduces a protocol with which to volatilize malondialdehyde
Reymond, P., Weber H., Damond M. and Farmer E.E. (2000) Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis. Plant Cell 12, 707-719. Details
This microarray analysis of responses to herbivory in Arabidopsis suggested an important role for jasmonates in gene expression following insect damage. Interestingly, genes that are strongly upregulated in mechanically crushed tissues and also induced during dehydration were less activated by insects. The implication is that insect feeding strategies might minimise the cut leaf surface exposed to the air. This, of course, might help explain why insects often cut circles and semi-circles in leaves.
New (March 2002): Selected as New Hot Paper by ISI Essential Science Indicator here
Farmer, E.E. (2000) Adding injury to insult:pathogen detection and responses. Genome Biology 1, reviews 1012.1-1012.3
Weber, H., Chételat, A., Caldelari, D. and Farmer, E.E. (1999) Divinyl ether fatty acid synthesis in late blight diseased potato leaves. Plant Cell 11, 485-493.
Many studies on antibiotics (phytoalexins) made in potato in response to the devastating pathogen Phytophora infestans have concentrated on tubers. We looked at the primary infection sites, leaves, and were surprised to find very high levels of fatty acid divinyl ethers which we describe as "phytoalexin-like compounds". Introduces the term "late-phase oxylipins" for oxylipins made or accumulating at late stages in pathogenesis.
Caldelari, D. and Farmer, E.E. (1998) Rapid assays for the coupled cell free generation of oxylipins. Phytochem 47, 599-604.
Reymond, P and Farmer, E.E. (1998) Jasmonate and salicylate as global signals for defense gene expression. Curr. Op. Plant Biol. 1, 404-411.
Weber, H., Vick, B.A. and Farmer, E.E. (1997) Dinor-oxo-phytodienoic acid: a new hexadecanoid signal in the jasmonate family. Proc. Natl. Acad. Sci. USA 94, 10473-10478. Introduces the concept of the "oxylipin signature" whereby more than one jasmonate family member is responsible for the fine-tuning of gene expression and that different jasmonates might play diferent biological roles. Presents a new method for the discovery and quantification of jasmonates and describes a new sixteen carbon jasmonate, dinor-OPDA.
Farmer, E.E. (1997) New fatty acid-based signals: A lesson from the plant world. (Perspective) Science 276, 912-913.