A Conserved Transcript Pattern in Response to a Specialist and a Generalist Herbivore

Supplemental Figure 1 - Analysis of variability in gene expression between control Arabidopsis plants. Volcano plot where gene expression ratios (log2 fold change) are plotted against the negative log10-transformed P values from a t test calculation. Expression ratios were obtained from six independent experiments of control versus control plants. Mean log2-transformed expression ratios were used for a t test (one samatple hypothesis, expression ratio not equal to 0). The vertical dashed lines represent a twofold threshold in gene induction or repression. The horizontal dashed line represents a significance level of P = 0.05.

Supplemental Figure 2 - Plot of the q values of the genes versus their respective P values. Results from five independent experiments of Arabidopsis plants challenged with Pieris rapae larvae were used to calculate a false discovery rate (q value) associated with each gene on the microarray (Storey and Tibshirani, 2003). One can see the proportion of false positives for different P value cutoffs from this plot.

Supplemental Figure 3 - Fatty acid analysis of regurgitant from Pieris rapae. Regurgitant from Pieris rapae larvae fed with wild-type (A) or fad3-2 fad7-2 fad8 triple mutant (B) plants was analyzed for the presence of linolenic (18:3) and linoleic (18:2) acid according to a published protocol (Weber et al., 1997).

Supplemental Table 1 - Mean expression ratios of genes induced after insect challenge, MeJA treatment ,and mechanical damage.

Supplemental Table 2 - Mean expression ratios of genes induced by Pieris rapae in local and distal leaves.

Supplemental Table 3 - Normalized signal intensities for genes listed in Supplemental Table 1.

Supplemental Table 4 - Normalized signal intensities for genes listed in Supplemental Table 2.

Supplemental Table 5 - Mean expression ratios of genes induced by Pieris rapae. Comparison of Col-0 and gl1 plants.