Université de Lausanne
Department de Biologie Moléculaire végétale
Tel: +41 (0)21 692 42 33
Defining molecular components of symbiotic phosphate uptake in Oryza sativa
Many plants obtain phosphate form soil according to the symbiosis with arbuscular mycorrhizal (AM) fungi. In this association, the fungi release phosphate form arbuscules, and plant can transport the phosphate across the periarbuscular membrane into the cortical cell. In Medicago truncatula, loss of MtPT4, a phosphate transporter located in the periarbulcular membrane, leads to premature death of the arbuscules (Javot et al., 2007). In rice we found that two rice phosphate transporters are up-regulated in the root colonized by the AM fungi (Paszkowski et al., 2002; Guimil et al., 2005). However, OsPT13 is strongly induced in the root colonized by Gigaspora rosea and only detected under very highly-mychorrhized roots colonized by Glomus intraradices. OsPT11 is strongly up-regulated in the root colonized by Gigaspora rosea and Glomus intraradices compared with that of non-colonized roots. It is very interesting to clarify how these two transporters respond different AM fungi and how they influence the AM symbiosis. In this purpose, I will knock-down two Pi transporters separately and collectively by making RNAi lines in order to investigate how they effect the association with Gigaspora rosea and Glomus intraradices. I also want to investigate the expression profiles and sub-cellular localizations of these two transporters.
Defining molecular components of symbiotic nitrogen uptake in Oryza sativa
In addition to phosphate, plants also obtain nitrogen from soil according to the symbiosis with arbuscular mycorrhizal (AM) fungi (Ames et al., 1983). The extraradical hyphae of AM fungi can absorb ammonium (NH4+) (Johansen et al., 1992, 1993, 1996), nitrate (NH3) (Johansen et al., 1996) and amino acids (Hodge et al., 2001) from the surroundings and translocate N to the plant. However, it is not clear that which components in plants play the most important role on the symbiotic nitrogen uptake. Some candidates such as mycorrhiza-induced ammonium transporters have been identified in the mycorrizal rice by using the transcriptome analysis (Guimil et al., 2005). It will be very interesting to identify the role of these transporters in symbiotic nitrogen uptake and the contribution of AM symbiosis to nitrogen uptake of plants.
PhD thesis: Defining molecular components of symbiotic phosphate and nitrogen uptake in Oryza sativa (preliminary title)
Sawers RJH, Yang SY, Gutjahr C, Paszkowski U (2008) The molecular components of nutrient exchange in arbuscular mycorrhizal interactions. In: Z.A. Siddiqui et al., (eds.), Mycorrhizae: Sustainable Agriculture and Forestry, pp. 37-59 ©2008 Springer, Dordrecht, The Netherlands.
2007/04~ present: PhD student in the University of Lausanne, Switzerland
2006/04~2007/03: research assistant for Research Fellow, Dr. Yue-Ie Hsing at the Plant and Microbial Biology Institution in Academia Sinica (R.O.C.). Investigation on the function of soybean LEA proteins.
2003/08~2006/04: research assistant for Assistant Research Fellow, Dr. Kuo-Chen Yeh at the BioAgricultural Sciences Preparatory Office in Academia Sinica (R.O.C.). Investigation on the function of Arabidopsis thaliana IRT3 and Arabidopsis halleri IRT3.
2001/09~2003/06: studied in Botany at the Nation Taiwan University and got the master degree. Diploma thesis with Dr. Tsan-Piao Lin on "Protection of membrane vesicles against desiccation by three LEA proteins (group Ⅳ) from soybean"
1997/09~2001/06: studied in Botany at the Nation Taiwan University and got the bachelor degree
Playing the piano, reading the novels, seeing the movies and art exhibition, mountain hiking.