Université de Lausanne
Department de Biologie Moléculaire végétale
Tel: +41 (0)21 692 42 33
The arbuscular mycorrhiza (AM) association results from a successful interaction between the majority of terrestrial plants and fungal symbiotic partners in the Glomeromycota. Host plants supply photosynthates to their AM fungal partners and, in return, AM fungi enhance the phosphate and nitrogen nutrition of their hosts. This bi-directional nutrient exchange is believed to take place at intracellular fungal structures, called the arbuscules, that form within the root cortical cells. Development of fungal arbuscules induces polarization of the colonized cell and is accompanied by the synthesis of an extensive periarbuscular membrane. This membrane remains in continuum with the peripheral plasma membrane and governs the molecular dialogue between fungus and plant. While there has been much progress in understanding signaling during the early phase of AM symbiosis development, the regulation of symbiosome development, and nutrient transport are largely unknown.
Proteomics is likely to be the best methodology to unravel the molecular components of the plant-fungus interactions. In a novel proteomics approach we aim at determining the molecular constituents of the periarbuscular membrane and the peripheral plasma membrane sub-domains of the polarized arbusculated cells.
Gutjahr C, Radovanovic D, Geoffroy J, Zhang Q, Siegler H, Chiapello M, Casieri L, An K, An G, Guiderdoni E, Kumar Chellian S, Sundaresan V, Harrison M, Paszkowski U (2012) The half-size ABC transporters STR1 and 2 are indispensable for mycorrhizal arbuscule formation in rice. Plant Journal DOI: 10.1111/j.1365-313X.2011.04842.x
Chiapello et al. Cellular response of Fusarium oxysporum to crocidolite asbestos as revealed by a combined proteomic approach. Journal of proteome research (2010)
Vallino, M., Martino, E., Boella, F., Murat, C., Chiapello, M., Perotto, S. (2009). Cu,Zn superoxide dismutase and zinc stress in the metal-tolerant ericoid mycorrhizal fungus Oidiodendron maius Zn. FEMS Microbiology Letters 293, 48-57.
A. Salvioli, M. Chiapello, J. Fontaine, A. Lounes Hadj-Sahraoui, A.Grandmougin-Ferjani, L. Lanfranco and P. Bonfante: “Endobacteria affect the metabolic profile of their host Gigaspora margarita, an arbuscular mycorrhizal fungus” Environmental Microbiology, IN PRESS