Specific recognition of mRNA targets by RNA binding proteins in Pseudomonas fluorescens CHA0.
Pseudomonas fluorescens CHA0 is a rhizosphere bacterium that protects the roots of host plants against pathogenic fungi by synthesizing diverse extracellular products such as antibiotics, exoproteases and hydrogen cyanide. The biosynthesis of these biocontrol compounds is strictly regulated by a "quorum sensing" two-component system, GacS/GacA.
At low cell density, the expression of the biocontrol compounds biosynthetic genes are repressed at the post-transcriptional level by two RNA binding proteins RsmA and RsmE. When the cellular density increases, the GacS/GacA system is activated and induces the transcription of three small noncoding regulatory RNAs (RsmX, Y and Z). These sRNAs capture the translational repressor proteins RsmA and RsmE thereby allowing the translation and production of the antifungal biocontrol compounds.
We are investigating the binding specificity between Rsm proteins and mRNAs of various biocontrol compounds indirectly regulated by GacA. We are also studying the binding equilibrium between the Rsm proteins-sRNAs and Rsm proteins-mRNA.
|Aug. 2008-||Assistant Professor at University of Lausanne, Fundamental Microbiology Department (Lausanne, Switzerland). Subject: Translational regulation governing the adaptation of Pseudomonas to environmental changes.
(Supported by the Sandoz Family Foundation)
|Mar. 2004-||Research Associate in the laboratory of Dr. D. Haas, University of Lausanne, Fundamental Microbiology Department (Lausanne, Switzerland). Subject: Study of regulatory elements in the quorum sensing network of Pseudomonas fluorescens.|
|Jan. 2003-||Research Associate in the laboratory of Dr. L. Borradori, University Hospital Geneva, Dermatology Department (Geneva, Switzerland). Subject: Study of proteins involved in the hemidesmosome and desmosome assembly.|
|Jan. 2000-||Research Associate in the laboratory of Dr. K. Rittinger, National Institute for Medical Research, Protein Structure (London, England). Subject: Study of the assembly of the NADPH oxidase.|
|Nov. 1999-||Post-doctoral research in the laboratory of Prof. R. van Grondelle, Vrije University (Amsterdam, The Netherlands). Subject: Electronic studies of modified light-harvesting 1 proteins. (FEBS 2 months short term fellowship)|
|1999||Ph.D.,University Paris-Sud XI (Orsay). Ph.D. performed in the Centre d'Études Atomique de Saclay, in the Laboratoire de Spectroscopie Raman des Protéines directed by Bruno Robert. Subject: Influence of the chemical nature of chlorophyll cofactors on the structure and function of the light-harvesting proteins of purple bacteria.|