Protein and Peptide Chemistry Facility (PPCF)
Catherine Servis: Head | What we offer | For more information
Catherine Servis: Head
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Catherine Servis received her PhD from the University of Heriot-Watt in Edinburgh on studies involving the localization, identification and synthesis of the immunodominant determinants of Lactate Dehydrogenase-B4 and IgG2a myeloma protein.After a postdoctoral training at the Basel Institute for Immunology (Hoffmann-La-Roche) she continued her training in protein chemistry at the Friedriech- Miescher Institute (Ciba Geigy-Novartis). In 1990, she joined the Institute of Organic Chemistry of the University of Lausanne where she was involved in the development of strategies for the solid phase synthesis of molecules for biological applications. She joined the Institute of Biochemistry in 1992 and she is heading the Protein and Peptide Chemistry Facility since then. |
What we offer
The objective of this facility is to provide information on selected aspects of peptide synthesis and protein structure and to create the resources to investigators to extend their capabilities on peptide synthesis, peptide/protein purification and mass spectroscopic analysis through the aid of protein chemists operating this facility.
SERVICES
The range of services provided by the Protein and Peptide Chemistry Facility are listed below:
Synthesis
- Peptide synthesis (linear or branched peptides)
- Peptide modification
- Peptide coupling to proteins
Purification
- Peptide purification
- Protein purification
Analysis
- Amino acid analysis
- Mass spectroscopic analysis
- Peptides
- Proteins
- Identification of naturally processed peptide antigens. Natural peptides extracted from antigen presenting cells by acid extraction are separated by HPLC and then identified by MS
- C-terminal sequencing. Peptides up to 3,000 da are digested by carbosypeptidases and the digestion products are identified by MS
- Identification and characterization of proteins
EQUIPMENT
Equipment currently in place include:
Peptide synthesis is performed using two automated Applied Biosystems 431A and 433A peptide synthesizers (up to 0.2 mmol) and an ACT348 OMEGA Advanced ChemTech multiple synthesizer (0.025 and 0.1 mmol), both employing Fmoc chemistry. Standard synthesis provides 20-100 mg of peptide with are delivered within two-three weeks. Quality control includes analytical HPLC and mass spectroscopic analysis.
High Performance Liquid Chromatography (HPLC) is performed by three Waters HPLC systems for analytical or preparative HPLC and a Hewlett Packard HPLC system for analytical applications.
Mass spectroscopic analysis is performed by a Voyager-DE Biospectrometry Workstation by Applied Biosystems and a 4700 Proteomics Analyzer MALDI- TOF/TOF by Applied Biosystems.
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