Aims and Scope
We propose a FENS-IBRO Training Centre covering high-resolution optical imaging techniques to study neural function in-vivo and in tissue preparations.
This 3-weeks course for PhD students and post-docs in neuroscience will consist of morning lectures by international and local faculty, progressing from principles of imaging techniques to important recent advanced applications like voltage-sensitive dye imaging, intrinsic optical signaling, in-vivo 2-photon imaging, light-sensitive ion-channel imaging and others.
Following the morning lectures the students will work on a scientific project (in small groups of 2-3) in the laboratories of the participating faculty, allowing them to gain hands-on experience with most of the aforementioned imaging techniques.
The evenings will be reserved for poster presentations, round table discussions on ethics and professional skills, and social activities.
The registration fee is 500 euro and it covers accommodation, meals and local transportation during the duration of the course.
A number of travel grants is available. Candidates must apply for a travel grant when they fill out their application on-line.
- Principles of light microscopy
- Principles of fluorescence and confocal laser scanning microscopy
- Advanced confocal microscopy/multi-colour excitation
- Reconstruction and retrospective analysis of neurons
- Principles of multi-photon excitation microscopy
- Correlative light- and electron microscopic analysis of neurons
- Fluorescent Ca2+ indicators and quantitative Ca2+ imaging in neurons
- Genetically encoded Ca2+ indicators
- Presynaptic Ca2+ imaging of cerebellar neurons
- In-vivo Ca2+ imaging of neurons with 2-photon laser-scanning microscopy
- FRET based imaging of 2nd messenger and kinase activation in neurons
- Total Internal Reflection microscopy (TIRF) for studying vesicle dynamics in neurons/glia cells
- Stimulated Emission Depletion microscopy (STED) microscopy for live imaging of neurons
- Voltage-sensitive dye imaging: Principles and in-vivo application
- Intrinsic signal optical imaging combined with in vivo 2-photon microscopy
- Long-term in vivo 2-photon microscopy of neuronal structure
- Ultra-structural analysis of imaged dendritic spines
- Laser scanning glutamate-uncaging-based mapping of synaptic connectivity
- Light-activated ion channels to stimulate neurons in-vitro and in-vivo
- Light-activated ion channel control of the reward system
Bezzi, P.; Bureau, I.; Chatton, J.-Y.; Cossart, R.; Decosterd, I.; Dodt, H.-U.; Garaschuk, O.; Griesbeck, O.; Helmchen, F.; Holtmaat, A.; Huebener, M.; Knott, G.; Lebrand, C.; Llano, I.; Muller, D.; Naegerl, V.; Oertner, T.; Petersen, C.; Schneggenburger, R.; Spring, K. and Welker, E.
Bezzi, P.; Broillet, M.-C.; Carleton, A.; Chatton, J.-Y.; Hill, S.; Holtmaat, A.; Hornung, J.-P.; Kiss, J.; Knott, G.; Lebrand, C.; Lüscher, C.; Lüthi, A.; Magistretti, P.; Marquet, P.; Muller, D.; Petersen, C.; Schneggenburger, R.; Seitz, A.; Volterra, A. and Welker, E.
We invite PhD students and post-docs in neuroscience to apply.