Lemanic Neuroscience
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Introduction to fluorescence imaging for the analysis of living cells

Organizer: Jean-Yves Chatton




Schedule 2014
Courses take place on Tuesdays, from 12:15 to 14:00.

07 January Basics of transmitted light and fluorescence microscopy
14 January Confocal microscopy
21 January Modes of image formation, acquisition, signal sampling
28 January

Dynamic recording of cellular functions by fluorescence imaging.
Intracellular calcium, pH, and sodium and their application to cellular signaling.
Problems related to imaging of living cells.

4 February Other optical applications (proposed topics):
Fluorescence recovery after photobleaching (FRAP), flash photolysis, multiphoton microscopy, evanescent wave microscopy, laser tweezers, fluorescence resonance energy transfer (FRET),
digital holography microscopy, optical contrasting methods (phase contrast, DIC).




Petit Auditoire de l'Ecole de Médecine, DBCM, Rue du Bugnon 9, Lausanne.



  • Lectures will be given in English.
  • In parallel with theoretical lectures, demonstrations will be proposed on the confocal microscopes of the Cellular Imaging Facility (details and registration on site).



Please register until December 15 by mail to lndscourses@gmail.com  or the course organizer mentioning the course title as subject and your supervisor in copy (on-site registration will be possible, too).



Course attendance and a multiple choice exam.

 in this site:

FBM Doctoral School - Amphipôle 316 - CH-1015 Lausanne  - Switzerland  -  Phone +41 21 692 40 14