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Introduction to fluorescence imaging for the analysis of living cells

Organizer: Jean-Yves Chatton

 

1 ECTS

 

Schedule
Courses take place on Tuesdays, from 12:15 to 14:00.

07 January 2014 Basics of transmitted light and fluorescence microscopy
14 January 2014 Confocal microscopy
21 January 2014 Modes of image formation, acquisition, signal sampling
28 January 2014

Dynamic recording of cellular functions by fluorescence imaging.
Intracellular calcium, pH, and sodium and their application to cellular signaling.
Problems related to imaging of living cells.

4 February 2013 Other optical applications (proposed topics):
Fluorescence recovery after photobleaching (FRAP), flash photolysis, multiphoton microscopy, evanescent wave microscopy, laser tweezers, fluorescence resonance energy transfer (FRET),
digital holography microscopy, optical contrasting methods (phase contrast, DIC).

 

 

Location

Petit Auditoire de l'Ecole de Médecine, DBCM, Rue du Bugnon 9, Lausanne.

 

Remarks

  • Lectures will be given in English.
  • In parallel with theoretical lectures, demonstrations will be proposed on the confocal microscopes of the Cellular Imaging Facility (details and registration on site).

 

Registration

Please register until December 15 by mail to the LN coordinator or the course organizer (on-site registration will be possible, too).

 

Evaluation

Course attendance and a multiple choice exam.
 


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FBM Doctoral School - Biophore 2204 - CH-1015 Lausanne  - Switzerland  -  Phone +41 21 692 40 14