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Research & Publications

RESEARCH

Research topics are :

  • those of the partners (see present and past partners)
  • the one of the head of qMSF : Role and Importance of Hepatic and Extrahepatic Drug Metabolizing Enzymes in the Bioavailability of Anti-Cancer Agents in Cancer Cells. See details in the document below or the following publication:  Rochat B. Role of Influx and Efflux Systems and Xenobiotic Metabolizing Enzymes in the Intratumoral Disposition of Anti-Cancer Agents. Curr Cancer Drug Targets. Curr Cancer Drug Targets. 2009;9:652-74.

    All financial supports are welcome.

PUBLICATIONS

Original Papers (peer reviewed)

Original Papers (peer reviewed; underlined = corresponding author).

1. Dahmane E, Boccard J, Csajka C, Rudaz S, Décosterd L, Genin E, Duretz B, Bromirski M, , Leyvraz S, Zaman K, Testa B and Rochat B. Quantitative monitoring of tamoxifen in human plasma extended to forty metabolites using LC-HR-MS: New investigation capabilities for clinical pharmacology. Submitted 2013

2. Sobhi HR, Rochat B, Henry H, Bruce SJ, Kashtiaray A.Simple Measurement of Testosterone in male saliva samples using liquid-liquid microextraction followed by liquid chromatography-tanderm mass spectrometry. In press. Am. J. Anal Chem.

3. Rochat B, Peduzzi D, McMullen J, Favre A, Kottelat E, Favrat B, Tissot J.D., Angelillo-Scherrer A, Bromirski M and Waldvogel S. Hepcidin quantification in plasma using liquid chromatography coupled to high resolution mass spectrometry. In Press 2013

4. Rochat B, Favre E and Sottas PE. Metabotype analysis for personalized biology: a new bioanalytical territory for high-resolution MS. Bioanalysis. 2013; 5: 1149-1152.

5. Glauser G, Veyrat N, Rochat B, Wolfender JL, Turlings TC. Ultra-high pressure liquid chromatography-mass spectrometry for plant metabolomics: A systematic comparison of high-resolution quadrupole-time-of-flight and single stage Orbitrap mass spectrometers. J Chromatogr A. 2013; 1292:151-9.

6. Bruce SJ, Rochat B, Béguin A, Pesse B, Guessous I, Boulat O, Henry H. Analysis and quantification of vitamin D metabolites in serum by ultra-performance liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry - a method comparison and validation. Rapid Commun Mass Spectrom. 2013;27:200-6.

7. Vale Silva L, Sanguinetti M, Vandeputte P, Torelli R, Rochat B, Sanglard D. Milbemycins: more than efflux inhibitors for fungal pathogens. Antimicrob Agents Chemother. 2012.

8. Rochat B, Quantitative/qualitative analysis using LC-HRMS: the fundamental step forward for clinical laboratories and clinical practice. Bioanalysis. 2012;4:1709-11.

9. Henry H, Sobhi HR, Scheibner O, Bromirski M, Nimkar SB, Rochat B. Comparison between a high-resolution single-stage Orbitrap and a triple quadrupole mass spectrometer for quantitative analyses of drugs. Rapid Commun Mass Spectrom. 2012;26:499-509.

10. Sobhi HR, Vatansever B, Wortmann A, Grouzmann E, Rochat B. Generic approach for the sensitive absolute quantification of large undigested peptides in plasma using a particular liquid chromatography-mass spectrometry setup. J Chromatogr A. 2011;1218:8536-43.

11. Kashtiaray A, Hadi Farahani H, Farhadi S, Bertrand Rochat B, Hamid Reza Sobhi HR. Trace Determination of Tamoxifen in Biological Fluids Using Hollow Fiber Liquid-Phase Microextraction Followed by High-Performance Liquid Chromatography-Ultraviolet Detection. AJAC. 2011; 2:429-436

12. Rochat B, Pascual A, Pesse B, Lamoth F, Sanglard D, Decosterd LA, Bille J, Marchetti O. Ultra-performance liquid chromatography mass spectrometry and sensitive bioassay methods for quantification of posaconazole plasma concentrations after oral dosing. Antimicrob Agents Chemother. 2010;54:5074-81.

13. Decosterd LA*, Rochat B*, Pesse B, Mercier T, Tissot F, Widmer N, Bille J, Calandra T, Zanolari B, Marchetti O. Multiplex ultra-performance liquid chromatography-tandem mass spectrometry method for simultaneous quantification in human plasma of fluconazole, itraconazole, hydroxyitraconazole, posaconazole, voriconazole, voriconazole-N-oxide, anidulafungin, and caspofungin. Antimicrob Agents Chemother. 2010;54:5303-15.

14. Vatansever B, Lahrichi S, Thiocone A, Salluce N, Matthieu M,Grouzmann E and Rochat B. Comparison between a linear Ion Trap and a Triple Quadrupole MS in the Sensitive Dectection of Large Peptides at Femtomole Amounts on Column. J. Sep Sci. 2010; 33: 1-11.

15. Abid K, Rochat B, Lassahn PG, Stocklin R, Michalet S, Brakch N, Aubert JF, Vatansever B, Tella P, De Meester I, Grouzmann E. Kinetic study of neuropeptide Y (NPY) proteolysis in blood and identification of NPY3-35, a new peptide cleaved by plasma kallikrein. J Biol Chem. 2009; 284:24715-24724.

16. Haouala A, Zanolari B, Rochat B, Montemurro M, Zaman K, Duchosal MA, Ris HB, Leyvraz S, Widmer N, Decosterd LA. Therapeutic Drug Monitoring of the new targeted anticancer agents imatinib, nilotinib, dasatinib, sunitinib, sorafenib and lapatinib by LC tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877:1982-1996.

17. Michod D, Annibaldi A, Schaefer S, Dapples D, Rochat B, Widmann C. Effect of RasGAP N2 Fragment-Derived Peptide on Tumor Growth in Mice. J Natl Cancer Inst. 2009;101:828-32.

18. Fayet A, Béguin A, Zanolari B, Cruchon S, Guignard N, Telenti A, Cavassini M, Günthard HF, Buclin T, Biollaz J, Rochat B, Decosterd LA. A LC-tandem MS assay for the simultaneous measurement of new antiretroviral agents: Raltegravir, maraviroc, darunavir, and etravirine. J Chromatogr B. 2009; 877:1057-69.

19. Rochat B, Fayet F, Widmer N, Lahrichi S, Pesse B, Décosterd L, and Biollaz J. Imatinib metabolite profiling in parallel to imatinib quantification in plasma of treated patients using Liquid chromatography - mass spectrometry. J Mass Spectrom 2008;43:736-52.

20. Rochat B, Zoete V, Grosdidier A, von Grünigen S, Marull M and Michielin O. In Vitro Biotransformation of Imatinib by the Tumor Expressed CYP1A1 and CYP1B1. Biopharm Drug Dispos. 2008;29:103-18.

21. Rochat B, Bolay S, Pascual A, Calandra T and Marchetti O. Liquid Chromatography - Mass Spectrometry Method for Quantification of Caspofungin in Clinical Plasma Samples. J Mass Spectrom. 2007;42: 440-449.

22. Colombo S, Buclin T, Franc C, Guignard N, Khonkarly M, Tarr PE, Rochat B, Biollaz J, Telenti A, Decosterd LA, Cavassini M. Ritonavir-boosted atazanavir-lopinavir combination: a pharmacokinetic interaction study of total, unbound plasma and cellular exposures. Antivir Ther. 2006;11:53-62.

23. Widmer N, Decosterd, LA, Csjaka C, Leyvraz S, Duchosal, MA, Rosselet A, Rochat B, Eap CB, Henry H, Biollaz, J, Buclin T. Population pharmacokinetics of imatinib and the role of alpha-acid glycoprotein. Br J Clin Pharmacol. 2006;62:97-112.

24. Marull M and Rochat B. Fragmentation Study of Imatinib and Characterization of New Imatinib Metabolites by Liquid Chromatography – Triple Quadrupole and Linear Ion Trap Mass Spectrometers. J Mass Spectrom. 2006;41:390-404.

25. Neef M, Ledermann M, Saegesser H, Schneider V, Widmer N, Decosterd LA, Rochat B, Reichen J. Oral imatinib treatment reduces early fibrogenesis but does not prevent progression in the long term. J Hepatol. 2006;44:167-75.

26. Colombo S, Beguin A, Telenti A, Biollaz J, Buclin T, Rochat B, Decosterd LA. Intracellular measurements of anti-HIV drugs indinavir, amprenavir, saquinavir, ritonavir, nelfinavir, lopinavir, atazanavir, efavirenz and nevirapine in peripheral blood mononuclear cells by liquid chromatography coupled to tandem mass spectrometry . J Chromatogr B Analyt Technol Biomed Life Sci. 2005;819:259-76.

27. Widmer N, Béguin A, Rochat B, Buclin T,Kovacsovics T, Duchosal M, Leyvraz S, Rosselet A, Biollaz J, Decosterd LA. Determiantion of imatinib (Gleevec) in human plasma by HPLC coupled with UV-Diode Array detection and Solid Phase Extraction. Journal of Chromatography B. 2004;803:285-292.

28. Toso C., Oberholzer J., Ceausoglu I., Morel Ph., Rochat B., Ris F., Rehor A., Triponez F., Bucher P., Wandrey C., Schuldt U., Belenger J., Lou J., Majno P., Hunkeler D.. Intraportal injections of 400m multicomponent microcapsules in a large animal model- Transpl Int. 2003;16:405-10.

29. Rochat B. Evaluation of Recombinant Cytochromes P450 activity in Metabolic Pathways. Letter to the Editors. Drug Metabolism and Dosposition. 2003;31,145-146.

30. Kosel M, Gnerre C, Voirol P, Amey M, Rochat B, Bouras C, Testa B, Baumann P. In vitro biotransformation of the selective serotonin reuptake inhibitor citalopram, its enantiomers and demethylated metabolites by monoamine oxidase in rat and human brain preparations. Molecular Psychiatry. 2002;7:181-188.

31. Rochat B, Murray GI, Figg WD and McLeod HL. Role of CYP1B1 in biotransformation of anticancer agents ? J Pharmacol and Exp Ther. 2001;296:537-541.

32. Collie-Duguid ES, Johndton SJ, Powrie RH, Milano G, Etienne MC, Rochat B, Watson GC, McLeod HL. Cloning and initial characterization of the human DPYD gene promoter. Biochem biophys Res Commun. 2000;271:28-35.

33. Rochat B, Baumann P and Audus KL. Transport of the antidepressant citalopram across bovine brain endothelial cells. Brain Research. 1999;831:229-236.

34. Gervot L, Rochat B, Gautier JC, Bohnenstengel F, Kroemer H, de Berardinis V, Beaune P and de Waziers I. Human CYP2B6: Expression, inducibility and catalytic activities. Pharmacogenetics. 1999;9:295-306.

35. Rochat B, Kosel M, Boss G, Testa B, Gillet M and Baumann P. Biotransformation of the selective serotonin re-uptake inhibitor citalopram by monoamine oxidases in human liver. Biochemical Pharmacology.1998; 56:15-23.

36. Rochat B, Amey M, Gillet M, Meyer UA and Baumann P. Identification of cytochrome P450 isozymes involved in N-demethylation of citalopram enantiomers using human microsomes. Pharmacogenetics. 1997;7:1-10.

37. Bondolfi G, Chautems Cl, Rochat B, Bertschy G and Baumann P. Non-response to citalopram indepressive patients: Pharmacokinetic and clinical consequences of a fluvoxamine augmentation. Psychopharmacology. 1996;128(4):421-425.

38. Rochat B, Amey M, van Gelderen H, Testa B and Baumann P. Determination of enantiomers of citalopram, its demethylated and propionic acid metabolites in human plasma by HPLC. Chirality. 1995;7,389-395.

39. Rochat B, Amey M and Baumann P. Analysis of enantiomers of citalopram and its demethylated metabolites in plasma of depressive patients using chiral reverse phase liquid chromatography. Therapeutic Drug Monitoring. 1995;17,273-279.

Review Papers (peer reviewed)

40. Rochat B, Kottelat E and Justin McMullen J. The Future Key Role of High Resolution Mass Spectrometry in LC-MS Analyses in Clinical Laboratories: A Focus on Quantitative Analysis. Bioanalysis. 2012; 4:2939-58.

41. Rochat B. Role of Influx and Efflux Systems and Xenobiotic Metabolizing Enzymes in the Intratumoral Disposition of Anti-Cancer Agents. Curr Cancer Drug Targets. 2009;9:652-74.

42. Rochat B. Role of cytochrome P450 activity in the fate of anticancer agents and in drug resistance: focus on tamoxifen, paclitaxel and imatinib metabolism. Clin Pharmacokinet. 2005;44:349-66.

43. Baumann P and Rochat B. Comparative pharmacokinetics of selective serotonin reuptake inhibitors: a look behind the mirror. International Clinical Psychopharmacology. 1995;10,15-21.

 

Book Chapter (peer reviewed)

44. Rochat B and Audus K. Drug Disposition and Targeting. Tansport across the Blood Brain Barrier. Book Chapter. Pharm. Biotechnol.1999;12:181-200.

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