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Isolation of mouse embryonic stem cell line
The overall strategy is to put blastocysts of any transgenic strain into culture and allow them to continue growth, for a limited period, until they achieve a stage which is equivalent to the early post-implantation embryo. The embryonic portion is then dissociated and culture of the cells is continued under conditions designed to be amenable to cell growth while encouraging stem cells to maintain the undifferentiated phenotype.
After about two weeks, stable ES cell line can be established.