Phenotyping

Mission

The missions of the phenotyping service include the phenotyping of engineered mice strains, the development of protocol for specific antibodies, as well as training and advising members of the CIG on the different available technologies.

Activities

• Advice and collaboration on different CIG projects
• Development of protocol tailored to each antibody
• Training of CIG members on the different machines (Cryostat, Microtome, etc…)
• Training of CIG members in histology practice-Test/benchmark the newly released products and technologies
• Manage a stock of secondary antibodies for the phenotypinglaboratory and CIG members

Techniques

• Dissection and Fixation-Inclusion in paraffin and freezing
• Cutting of sections in paraffin and freezing
• Fluorescence and Enzymatic immunohistochemistry techniquesIn Situ Hybridization-Usual topographic colorations ( H&E, RN, MG…)
• Specific colorations (fat, elastic fibers, iron…)
• Acquisition of pictures in transmission and fluorescence with theZeiss Axio Imager A 1 Microscope

Equipment for all users

Room 2013

• 2x CRYO-STAR HM 560 M and HM 550 M
• 2x Manual MicrotomHM 310
• 2x Tissue embedding center MICROM 350 andLeicaEG1140H
• Automatic Tissue Processor LeicaTP1020
• SAKURA (mounting slides) and LeicaCV5030
• Microscope Zeiss Imager A1
• Gene Paint TECAN

For the equipment booking, press here

1. Endothelial, but not smooth muscle, peroxisome proliferator-activated receptor β/δ regulates vascular permeability and anaphylaxis
2. Urate-induced acute renal failure and chronic inflammation in liver-specific Glut9 Knockout mice
3. Major histocompatibility Class II Pathway Is Not Riquired for the Development of Nonalcoholic Fatty Liver Disease in Mice
4. PPARβ/δ affects pancreatic β cell mass and insulin secretion in mice
5. GW501516-activated PPARbeta/delta promotes liver fibrosis via p38-JNK MAPK-induced hepatic stellate cell proliferation
6. Epiblast-specific loss of HCF-1 leads to failure in anterior-posterior axis specification