The hyperglycemic clamp allows assessment of in vivo pancreatic b-cell function, i.e., glucose-induced insulin secretion. Five hour-fasted, awake and free-moving mice receive a glucose infusion through a femoral vein indwelling catheter. Infusion rates are adjusted in order to maintain the blood glucose concentration at either 10 or 20 mM, as determined from tail vein blood. After 180 min of glucose clamp, blood is collected for measurement of plasma insulin levels.
Ten to 12 mice per group should be sent to our facility, one week prior to the experiment. In some cases, an additional, smaller cohort might be required to complete the experiment.
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Burcelin R, Thorens B. Evidence that extrapancreatic GLUT2-dependent glucose sensors control glucagon secretion. Diabetes. 2001 Jun;50(6):1282-9
Thorens B, Guillam MT, Beermann F, Burcelin R, Jaquet M. Transgenic reexpression of GLUT1 or GLUT2 in pancreatic beta cells rescues GLUT2-null mice from early death and restores normal glucose-stimulated insulin secretion. J Biol Chem. 2000 Aug 4;275(31):23751-8.