To study the counterregulatory response to hypoglycemia, 6 hour fasted, awake and freely moving mice are subjected to hyperinsulinemic (18 mU · kg–1 · min–1) -hypoglycemic (2-2.5 mmol/l) clamps. A glucose solution is co-infused with insulin to maintain blood glucose levels at the target value during the experimental period. After completion of the infusions, blood is collected for determination of plasma levels of counterregulatory hormones (e.g. glucagon).
Ten mice should be sent to our facility, one week prior to the experiment. In some cases, an additional, smaller cohort might be required.
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Emery M, Nanchen N, Preitner F, Ibberson M, Roduit R. Biological Characterization of Gene Response to Insulin-Induced Hypoglycemia in Mouse Retina. PLoS One. 2016 Feb 26;11(2):e0150266. doi: 10.1371/journal.pone.0150266.
Emery M, Schorderet DF, Roduit R. Acute hypoglycemia induces retinal cell death in mouse. PLoS One. 2011;6(6):e21586.
Burcelin R, Thorens B. Evidence that extrapancreatic GLUT2-dependent glucose sensors control glucagon secretion. Diabetes. 2001 Jun;50(6):1282-9
Thorens B, Guillam MT, Beermann F, Burcelin R, Jaquet M. Transgenic reexpression of GLUT1 or GLUT2 in pancreatic beta cells rescues GLUT2-null mice from early death and restores normal glucose-stimulated insulin secretion. J Biol Chem. 2000 Aug 4;275(31):23751-8.