Indwelling venous catheters can be used for various infusions including tracers, metabolic substrates, peptides, drugs, etc. Arterial catheters are used for blood sampling in a non-stressing condition. Catheters are placed under anesthesia, after loss of pedal and corneal reflexes is assured. Mice are allowed to recover for 4-7 days and only mice that have regained >95% of their preoperative weight are studied.
Number of mice will depend on the experimental protocol and should be discussed. Subsequent experiments should be best performed in our facility.
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