Immune Landscape Laboratory Platform

| Mission | Offer | Clients | Instruments
 

IN SCREEN image web 1200 x 200 (7).png

Mission

The mission of the Immune Landscape Laboratory (ILL) platform is to assist researchers in state-of-the-art analysis of the tumor microenvironment, using fluorescence multiplex imaging and digital spatial profiling.

Such a multimodal approach is expected to provide high resolution, spatial and molecular picture of global tumor architecture and interrelations of its individual components, such as immune and cancer cells, fibroblasts, endothelial cells or extracellular matrix. Coupled with advanced bioinformatics analyses, the results will be important for identification of biomarkers of treatment responses.

The ILL platform is a part of the Centre for Experimental Therapies and as such, part of the Department of Oncology UNIL CHUV.

TOP ^

Offer

The ILL platform offers a tissue-processing platform where FFPE sample are freshly cut for further processing, and four analytic platforms :

  1. Multiplex Immunofluorescence platform for 7-plex staining and high-definition whole slide scanning of FFPE sections and detailed analyses with dedicated software.
  2. RNAscope, Basescope and miRNAScope on FFPE sections with detailed analyses of specific RNA targets in situ, from mRNA, specific TCR and micro RNA.
  3. Laser Capture Microdissection and RNA extraction and profiling on Ncounter (Nanostring)
  4. Digital Spatial profiling of Protein (91-plex) and RNA (1800 to 18000 targets) on FFPE sections (GeoMxDSP, Nanostring).

TOP ^

Clients

Customers of the ILL platform include scientists in academic research laboratories, large non-profit clinical trials, and pharmaceutical companies worldwide.

TOP ^

Instruments

Ventana Discovery Ultra autostainer (ROCHE)

For multiplex IF staining up to 30 slides at once.

Vectra3 and Vectra Polaris scanner (AKOYA)

For whole slide high-definition multiplex images.

mIF quantification using Inform software (AKOYA) and in House IFQuant software

 

Laser Capture Microdissection (Leica)

 

Ncounter and GeoMx DSP (Nanostring)

For digital spatial profiling.

TOP ^

Follow us:  

CONTACT

Directors

DO_Pls-0407-crop388x509.jpg

Pr George COUKOS

Oncology Lausanne Kandalaft Lab-crop

Pr Lana KANDALAFT

General managers

DO_Pls-0201-crop380x456.jpg
Dr Sylvie RUSAKIEWICZ

Oncology Lausanne ILL Stephanie Tissot-crop1659x2431.jpeg

Dr Stéphanie TISSOT

Email

Phone +41 21 314 64 53

Ch. des Boveresses 155 - CH-1066 Epalinges
Switzerland
Tel. +41 21 692 59 92
Fax +41 21 692 59 95
Ludwig Cancer Research Université de Lausanne Centre Hospitalier Universitaire Vaudois (CHUV)