Technology

From UnTargeted discovery to high-coverage Targeted metabolome and lipidome quantification

Our platform is equipped with two high resolution LC-HRMS systems (Agilent 6550 iFunnel QTOF and Thermo QExactive Orbitrap) and three tandem LC-MS/MS systems (Sciex QTRAP 6500 plus, Thermo TSQ Altis and Agilent 6495 iFunnel QQQ).

We enjoy UnTargeted and appreciate the power of Targeted (in an absolute manner of course!)

Agilent LC-MS 6550 iFunnel Q-TOF system

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The Agilent LC-MS 6550 iFunnel Q-TOF is a high resolution system well suited for qualitative and quantitative metabolome and lipidome analyses. It oncorporates the Agilent Jet Stream thermal gradient focusing technology for improved sensitivity. Its TOF technology enables the data acquisition in full mass range mode (m/z 25 to 10,000) and delivers exceptional mass accuracy (<1ppm) and resolution (>20,000). High data acquisition rates of up to 50 spectra/second allow for maximum data collection during data-dependent (DDA) and data-independent (DIA) MS/MS experiments and thus facilitate metabolite identification in untargeted metabolomic experiments. Instrument is interfaced with Agilent 1290 Infinite UHPLC modules and equipped with ESI source.

ThermoFisher Scientific LC-MS Q Exactive™ Hybrid Quadrupole-Orbitrap™ system

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The QExactive Quadrupole Orbitrap is a high performance hybrid quadrupole mass spectrometer combining the high-field OrbitrapTM mass analyzer with a quadrupole precursor ion selection with high resolution (up to 140 000) and accuracy (< 1ppm) that can be achieved routinely. Data can be acquired in full-scan and all-ion fragmentation (AIF in-source CID and HCD) modes to ensure confident metabolite identification. The Q Exactive system is equally useful for untargeted and targeted screening in metabolomics. The instrument is interfaced with Thermo Accela 1250 UPLC pump and CTC PAL Analytics autosampler.

Sciex LC-MS/MS QTRAP 6500 plus system

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Sciex LC-MS QTRAP 6500 plus system is a highly sensitive and wide dynamic range instrument that we have dedicated for targeted quantification of complex lipids. It can be operated in MS scan, MS/MS product ion-scan, MRM, neutral loss/gain, and precursor ion scan modes (mass range spans from 30-2000 Da, scan speed up to 20000 Da/sec). It is equipped with an IonDrive Turbo V-source ESI (interchangeable APCI) offering the most efficient ionization at standard UPLC flow rates. This source can be upgraded with SelexION technology for more selective and powerful separation of isobaric lipid species. The MS is interfaced with Exon UHPLC modules. Due to improved scanning speed, several hundreds of lipids can be quantified in a scheduled MRM (or dynamic MRM) mode in a single run with optimal sensitivity and reproducibility.

 

Thermo LC-MS/MS TSQ Altis Triple Quadrupole system

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Thermo LC-MS/MS TSQ Altis system is a highly sensitive and wide dynamic range instrument that we have dedicated for targeted quantification of polar metabolites. It can be operated in MS scan, MS/MS product ion-scan, MRM, neutral loss/gain, and precursor ion scan modes (mass range spans from 5 -2000 Da, scan speed up to 600 SRMs/sec). It is equipped with an OptaMax NG ESI ion source (interchangeable APCI) offering the efficient ionization at standard UPLC flow rates. The MS is interfaced with Vanquish UHPLC modules. Due to high scanning speed and extra selectivity it allows for several hundreds of polar metabolites to be quantified in a scheduled MRM (or dynamic MRM) mode in a single run with optimal sensitivity and reproducibility.

Agilent LC-MS 6495 Triple Quadrupole system

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Agilent 6495 LC-MS QqQ system is a highly sensitive and wide dynamic range instrument dedicated for targeted quantification of metabolites (and lipids) of interest. It can be operated in MS scan, MS/MS product ion-scan, MRM, neutral loss/gain, and precursor ion scan modes (mass range spans from 30-2250 m/z, scan speed up to 15000 u/sec). It is equipped with an Agilent Jet Stream ESI (interchangeable APCI) source offering the most efficient ionization at standard UPLC flow rates. The QqQ is interfaced with Agilent 1290 Infinite UHPLC modules. The possibility to schedule MRM (dynamic MRM) simplifies method development and allows several hundreds of metabolites to be quantified in a single run with optimal sensitivity and reproducibility.

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Quartier UNIL-CHUV - Rue du Bugnon 19 - CH-1005 Lausanne
Switzerland
Tel. +41 21 692 50 98