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Introduction

The PAF (Protein Analysis Facility) is a service and research laboratory specialized in the analysis of proteins and proteomes.

The primary mission of the PAF is to support the local academic community and to function as a catalyst in the application of advanced technologies to analyse protein expression and function.

The PAF is affiliated to the CIG (Center of Integrative Genomics). The platform has been created in 2002 thanks to contributions from the University of Lausanne and a very generous donation by the Leenardts Foundation. Service activities are:

  • advisory and technical support for local groups that want to undertake proteomics projects.
  • protein separation and profiling by one and two-dimensional electrophoresis.
  • protein identification and quantification by mass spectrometry in simple or complex mixtures (shotgun proteomics).
  • analysis of post-translational modifications (for example phosphorylation).
  • targeting analysis of proteins and phosphoproteins by Reversed Phase Protein Arrays (RPPA)

Goals of research and development

  • to implement new technologies for the qualitative and quantitative comparative analysis of proteomes.
  • collaborate with local investigators on selected biological research projects.

Organisation

The PAF is located in Genopode building (lab 1018) on the Dorigny campus.

A Steering Committee has been designated to supervise the PAF functioning and take strategic decisions on the development of the platform. Its members are :

Prof. Alexandre Reymond (CIG, Director)
Prof. Nicolas Fasel (Dept. Biochemistry)
Dr. Gilbert Greub (CHUV)
Dr. Jean-Charles Sanchez (UNI Geneve)
Dr. Marc Moniatte (EPFL)
Dr. Jérome Wuarin (Decanat FBM)

The PAF is also closely linked to similar units at the EPFL (PCF) and University of Geneva (PCF).

Teaching

We provide courses at the master and postgraduate level to disseminate the knowledge of proteomics technologies among students and researchers.

Networking

Since August 2010, the PAF is member of the RéPP-SO ("Réseau des Plate-formes de Protéomique de Suisse Occidentale"), a network of proteomics facilities in West Switzerland.

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