Peptidomics

Determination of the peptidome

The peptidome is composed of peptides (very small proteins <10kDa) and is more and more recognized as a significant part of biological systems beside genomics, transcriptomics, proteomics and metabolomics. Indeed, peptides are widely represented among the signaling molecules in living systems.

Peptides are synthesized from protein-precussors (pro-peptides) or directly from mRNA. Peptides are hormones, neuromodulators, antibiotics, agonists or antagonists, inflammatory mediators etc. The determination of peptides with liquid-chromatography mass-spectrometry is crucial to study their action and fate with the detection specificity needed.

We have developed a new and original LC-MS methodology to discover, identify and quantify endogenous peptides. The method has benefit from previous peer-reviewed articles (see below) and expertise. The discovery of new peptides from plants or animals has become of high interests in order to find new therapeutics (often a copy-paste of their structure with some chemical modifications).

Hashimoto Y et al. Dev Growth Differ. 2008.

Publications

  • Rochat B. From targeted quantification to untargeted metabolomics: Why LC-high resolution-MS will become a key instrument in clinical labs. Trends Analyt Chem. 2016; 81:151-164.
  • Waldvogel S, Rochat B, Peduzzi D, Vaucher P, Tissot JD, Favrat B. Effects of oral supplementation of iron on hepcidin blood concentrations among non-anaemic female blood donors: a randomized controlled trial. Vox Sang. 2016;110:166-71.
GĂ©nopode - CH-1015 Lausanne
Switzerland
Tel. +41 21 692 56 76
Fax +41 21 692 57 05