Determination of the peptidome
The peptidome is composed of peptides (very small proteins <10kDa) and is more and more recognized as a significant part of biological systems beside genomics, transcriptomics, proteomics and metabolomics. Indeed, peptides are widely represented among the signaling molecules in living systems.
Peptides are synthesized from protein-precussors (pro-peptides) or directly from mRNA. Peptides are hormones, neuromodulators, antibiotics, agonists or antagonists, inflammatory mediators etc. The determination of peptides with liquid-chromatography mass-spectrometry is crucial to study their action and fate with the detection specificity needed.
We have developed a new and original LC-MS methodology to discover, identify and quantify endogenous peptides. The method has benefit from previous peer-reviewed articles (see below) and expertise. The discovery of new peptides from plants or animals has become of high interests in order to find new therapeutics (often a copy-paste of their structure with some chemical modifications).