Western in capillaries

Principle

All steps are fully automated inside independant capillaries. Jess from ProteinSimple

Adapted from ProteinSimple

Numbers

  • 24 independant antibody/samples combinations per run
  • 0.6 - 1.2 µg sample/lane
  • 3 µl sample/lane
  • 10 µl antibody dilluted solution/lane
  • Molecular weight ranges from 2-440 kDa
  • Only 1hr hands-on time
  • 3hr30 run
  • Detection by chemiluminescence and/or fluorescence (Near Infra Red or InfraRed)

As compared to western blot

6 samples were tested with 4 different antibodies using either one run of Jess or 2 SDS-PAGE followed by western blotting(wet transfer).

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