Instrumentation

Thermo Scientific Orbitrap Fusion | Thermo Scientific Q Exactive Plus | Thermo Scientific LTQ-Orbitrap Velos | RPPA instrumentation | Separation | 2D gel equipment
 

Thermo Scientific Orbitrap Fusion

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This mass spectrometer is the latest generation of Orbitrap system with a "Tribrid architecture" combining the best of quadrupole, Orbitrap and linear ion trap mass analyzers.
This versatile instrument is used for shotgun proteomics, either in data-dependent or data independent mode. It is installed with an ETD fragmentation system, particularly suitable for the analysis of post-translational modifications and large peptides/intact proteins. This mass spectrometer is interfaced to an Ultimate 3000 Nano LC System from Dionex.

Thermo Scientific Q Exactive Plus

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This instrument is a hybrid quadrupole-Orbitrap mass spectrometer acquiring fast MS and MS/MS spectra at high resolution. It is used for shotgun qualitative/quantitative proteomics and is interfaced to an Ultimate 3000 Nano LC System from Dionex.

Thermo Scientific LTQ-Orbitrap Velos

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This mass spectrometer is an hybrid composed of a dual-pressure linear ion trap and an electrostatic Fourier Transform (FT) analyzer, the Orbitrap. Its main features are very high resolution (up to 100'000), speed (up to 3 MS/MS spectra per second) and sensitivity (attomole levels). This instrument is used for shotgun qualitative/quantitative proteomics and is interfaced to an Ultimate 3000 Nano LC System from Dionex.

RPPA instrumentation

Slide spotter: Nano-Plotter 2.1 (GeSiM)

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Slide incubations: Biomek 3000 (Beckman-Coulter)

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Slide detection: InnoScan 710 (Innopsys)

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Separation

AKTA Purifier FPLC: for LC purification of proteins and fractionation of tissue and cell extracts

OFFGel: for separation of peptides after protein digestion

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2D gel equipment

The 2D-PAGE equipment of the PAF in Epalinges includes :

Minigels 7 x 9 cm : GE / Hoefer SE 250
1D separations and 2D-PAGE (mostly for optimization or Western blotting)


Midigels 9 x 13 cm : Bio-Rad Criterion system (precast gels)
2D-PAGE for medium complexity systems or preliminary experiments


Imaging:

GS-710 densitometer (Bio-Rad) : visible light scanner for Coomassie Gels, silver.

FX-Pro Molecular Imager (Bio-Rad) : scanning at 3 laser wavelengths.

Génopode - CH-1015 Lausanne
Switzerland
Tel. +41 21 692 56 76
Fax +41 21 692 57 05