Jean-Yves Roignant carried out his thesis in the laboratory of Dr. Jean-Antoine Lepesant at the Jacques Monod Institute and received his Ph.D. in 2003 from the University of Paris Diderot. He became postdoctoral fellow and research associate in the group of Dr. Jessica Treisman at New York University Langone Medical Center. He then started his independent group in 2012 at the Institute of Molecular Biology in Mainz and joined the Center for Integrative Genomics in August 2019 where he was appointed Associate Professor. He has a long-standing interest in the regulatory mechanisms of gene expression.
Keywords: RNA modifications, epitranscriptomics, gene expression, animal development, behavior
Regulation of gene expression by RNA modifications
A fundamental question in biology is to understand how gene expression is finely regulated in time and space during organismal development and function. While the influence of transcription regulation in these processes has been thoroughly studied the role of post-transcriptional gene regulation has emerged more recently as a critical player. Analog to epigenetic marks on the DNA, cellular RNAs are naturally decorated with a variety of chemical modifications that are often highly conserved and can be dynamically regulated upon environmental changes. Recent advances in sequencing methods and mass spectrometry technologies uncovered the prevalence and importance of some of these modifications in biological function and diseases, resulting in the birth of the epitranscriptomics field. Yet, the biological function of the majority of modified RNA nucleotides and their molecular mechanisms on gene expression still remain to be investigated.
Our research goal strives to unveil the epitranscriptome. To tackle this fundamental question we use Drosophila as a powerful model organism to study animal development, behavior, and human genetic diseases and conditions. We combined genetic approaches with state of the art molecular biology, biochemistry, microscopy, high throughput techniques and computational tools. Our research focuses on three main axes:
- Identification and mapping RNA modifications
- Elucidation of their biological and molecular functions
- Deciphering their evolutionary strategies and roles in diseases
For Masters, PhD and Postdoc position inquiries please write directly to
- Akhtar J, Kreim N, Marini F, Mohana GK, Binder H and Roignant JY (2019). Promoter proximal pausing mediated by the exon junction complex regulates splicing. Nat Commun, 10:521, doi:10.1038/s41467-019-08381-0
- de Brouwer* APM, Jamra* R, Körtel* N, Soyris C, Polla DL, Safra M, Zisso A, Powell CA, Rebelo-Guiomar P, Dinges N, Morin V, Stock M, Hussain M, Shahzad M, Riazuddin S, Ahmed ZM, Pfundt R, Schwarz F, de Boer L, Reis A, Grozeva D, Raymond FL, Riazuddin S, Koolen DA, Minczuk M, Roignant# JY, van Bokhoven# H, Schwartz# S (2018). Variants in PUS7 cause intellectual disability with speech delay, microcephaly, short stature, and aggressive behavior. Am J Hum Genet, 103:1045-1052, doi:10.1016/j.ajhg.2018.10.026.
- Knuckles P, Lence T, Haussman I, Jacob D, Kreim N, Carl SH, Masiello I, Hares T, Villasenor R, Hess D, Andrade-Navarro MA, Biggiogera M, Helm M, Soller M, Bühler# M and Roignant# JY (2018). Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to Wtap/Fl(2)d. Genes Dev, 32:415-429, doi: 10.1101/gad.309146.117
- Lence T, Akhtar J, Bayer M, Schmid K, Spindler L, Ho CH, Kreim N, Andrade-Navarro MA, Poeck B, Helm M and Roignant JY (2016). m6A modulates neuronal functions and sex determination in Drosophila. Nature, 540:242–247, doi: 10.1038/nature20568
- Malone CD, Mestdagh C, Akhtar J, Kreim N, Deinhard P, Sachidanandam R, Treisman JE, Roignant JY (2014). The exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript. Genes Dev 28:1786-1799, doi: 10.1101/gad.245829.114
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|Prof. Jean-Yves Roignant|
Praveen Bawankar - Post-doctoral fellow
Praveen obtained his PhD from the Indian Institute of Technology Bombay, in India, in the lab of Prof. Swati Patankar, where he identified and characterized the end modifications of small molecular weight RNAs in Plasmodium falciparum. Following his PhD, he carried out his post-doctoral research in the lab of Elisa Izaurralde at the Max Planck Institute for Developmental Biology Tübingen. There, he characterized the CCR4-NOT complex in humans and Drosophila melanogaster. He joined our group in June 2018 to characterize the m6A writer complex as well as the impact of m6A on post-transcriptional processes.
|Vishwaja Jhaveri - PhD Student
Vishwaja obtained her Bachelor and Master degrees in Cell and Molecular Biology from the Maharaja Sayajirao University of Baroda in India in 2018. She carried out her master’s thesis in Dr. Ravi Muddashetty’s group at the Institute of Stem Cell Science and Regenerative Medicine (inStem), Bangalore, where she investigated the mechanism of miRISC mediated translation repression in rodent neurons. Vishwaja continued as a Research Fellow with Dr. Muddashetty, working on neurodegeneration and neuronal activity mediated regulation of protein synthesis using human stem cells and rodent primary cell models, before joining our group in November 2020. She now wishes to understand the impact of RNA modifications on neurodegenerative diseases using Drosophila as model organism.
Guillaume Lavergne - Post-doctoral fellow
Guillaume obtained his Master in Genetics and Physiology at the University of Clermont-Ferrand in France in 2013. He then performed his PhD thesis in the Jagla lab at the GReD Institute in Clermont-Ferrand, where he studied muscle stem cell biology during Drosophila embryogenesis at the interface between stem cell biology, neurogenesis and myogenesis. Guillaume joined our group in January 2021 to elucidate the mechanisms underlying m6A regulation in Drosophila.
Iris Marouani - Administrative Assistant
Iris discovered the world of Science in 2000 when she took up a position as an accountant and HR manager at the Ludwig Institute for Cancer Research in Epalinges, Switzerland. After the integration of the Institute to the University of Lausanne in 2011 Iris pursued her career as an adminstration manager in a start-up focusing in the development of Tetramer. In 2016 Iris joined the Center for Integrative Genomics as an administrative assistant.
Chiara Paolantoni - PhD Student
Chiara obtained her Bachelor's degree in Biology from the Sapienza University of Rome in 2014, where she studied the regulation of Neurexin1 expression during neuronal differentiation. In 2016, she obtained her Master's degree in Neurobiology from the Sapienza University of Rome. During her degree, she worked in the lab of Dr. Corinna Giorgi at the European Brain Research Institute of Rome, studying how BDNF modulates the translation of dendritic Arc mRNA. Afterwards, she spent one semester investigating the role of Kif2a and Cep170 in microtubule organization in the lab of Dr. Jens Lüders at the Institute for Research in Biomedicine (IRB) of Barcelona, thanks to an Erasmus+ fellowship. In October 2017, she started her PhD in our group, initially at the Institute of Molecular Biology (IMB) in Mainz and then at the CIG. She was awarded a 2-year PhD fellowship from the Boehringer Ingelheim Fonds to study the molecular mechanisms of the m6A mRNA modification in Drosophila.
Athéna Sklias - Post-doctoral fellow
Athena obtained a bachelor in Biochemistry and Molecular Biology at the University of Strasbourg followed by a Master in Plant Production Management with a specialisation in Environmental Toxicology during which she studied the effect of pesticides on cell models and honeybees. To broaden her knowledge in bioinformatics, she obtained a Master’s degree in high-throughput technologies. She pursued into a PhD to study the estrogenic regulation in breast cancer at an epigenome-wide level in the group of Epigenetics at the International Agency for Research on Cancer (WHO) in Lyon, France. She joined our lab in January 2020 to explore the role of RNA modifications upon environmental changes and the regulation of non-coding RNAs.
Mariangela Spagnuolo - PhD Student
Mariangela obtained her Bachelor and Master degrees in Molecular and Cellular Biology from the University of Siena in Italy. During her degrees she worked at the University of Cambridge in Dr Olivia Casanueva’s and Dr Patrick Varga-Weisz’s laboratories, where she investigated the role of epigenetic modifications in the context of aging in C. elegans and of intestinal homeostasis in mice, respectively. She then moved to Mainz in Germany where she joined our group in 2018 to study the impact of RNA modifications on RNA metabolism in Drosophila melanogaster.
Michael obtained his Bachelor’s degree in Biology from University of Bayreuth in 2016, where he studied the effect of CapD2 mutations on the development of the larval brain of Drosophila under the supervision of Prof. Dr. Stefan Heidmann. He continued his studies to obtain his Masters in Biochemistry and Molecular Biology in 2018 during which he joined our group as a master student. He pursued his work as a PhD student since June 2018 focusing on the role of pseudouridine modification in Drosophila melanogaster.
Michaël Wiederkehr - Technician
Michaël obtained his Bachelor in Biotechnology from HES-SO Valais in Sion, Switzerland. Since 2011 he has worked in different groups in the CIG. He joined our lab in October 2019.
Lina Worpenberg - PhD Student
Lina obtained her Bachelor in Biochemistry from the Free University Berlin in 2014. During her degree, she joined for one semester the lab of Ville Hietakangas at the University of Helsinki to perform a systematic genetic analysis of genes involved in sugar metabolism of Drosophila melanogaster, and for her bachelor thesis, the Hamann lab at the DRFZ to identify immunoregulatory substances by using HT-Flowcytometry and expression analysis of cytocines. She continued with the Master studies in October 2014 at the Free University in Berlin, where she was working in the Pflueger lab to investigate the functional neuroanatomy and immunhistochemistry of the thoracal ganglia of insects, and, the Sigrist lab to perform systematic analysis of the function and location of Unc-13 isoforms at the neuromuscular junction. In 2015, she worked for a few months in the Ladant lab at the Pasteur Institute in Paris to help identifying the activator of Pseudomonas aeruginosa ExoY nucleotidyl cyclase and related ExoY-like effector domains from MARTX toxins. Afterwards, she joined the Grabowski lab at the Charite Berlin for the functional analysis and interactions of FoxM1 and Stat3 in gastroenteropancreatic neuroendocrine tumors. She joined our group in April 2016, first as a Master student and from 2017 on as a PhD student to investigate the m6A pathway in Drosophila melanogaster.
Our research is in the news, check it out!
D’un cerveau à l’autre : le diable est dans le méthyle! (http://insb.cnrs.fr/fr/cnrsinfo/dun-cerveau-lautre-le-diable-est-dans-le-methyle)
RNA modification important for brain function (https://www.sciencedaily.com/releases/2016/12/161208125812.htm)
Reading Sex-lethal (https://www.nature.com/articles/nchembio.2291)